The IC50 is the concentaion of your drug that reduces the viabilty of you cells by 50% (I just add this as in reply what was in the initial post ) If I am comparing IC50 values for viability honestly I do it manually by reading of the IC50 value for each individual experiment (not replicate just the average for each experiment). The problem with calculating IC50 values in Prism is that you must have proper dose response curves for it to work properly as in calculates based on a formula (hope I am making sense ) i.e if you viability curve does reach 0% it can cause problems. The IC50 values are determined using GraphPad Prism 6 with Sigmoidal 4PL equation. These results drastically change fold changes. The data points are fitted to Morrison equation using GraphPad Prism 6. Open Access Journal: Pharmacognosy Journal is an open access journal, which allows authors to fund their article to be open access from publication.I tried GraphPad Prism to calculate ic50, however it gives different results from the results of excel. Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online publication is 45 days.
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Pharmacognosy Journal (Phcog J.) covers different topics in natural product drug discovery, and also publishes manuscripts that describe pharmacognostic investigations, evaluation reports, methods, techniques and applications of all forms of medicinal plant researchĭistinctions: The most widely read, cited, and known Pharmacognosy journal and website is well browsed with all the articles published. Conclusions: The results of the present findings strengthen the potential property of Simarouba glauca as a resource for the discovery of novel antioxidant and anticancer agents. The results confirmed that ethanol extract significantly (p<0.05) inhibited T-24 cell line with IC50 value (533.55☒5.02 μg/mL) as compared to standard drug doxorubicin (0.16μM/mL). (B) Murine BRAFV600E YUMM1.7 melanoma cells and BRAFWT B16F10 melanoma cells were treated with. Ethanol and methanol extract showed highest antioxidant capacity in DPPH, FRAP and PM assay as compared to the other extracts based on the test performed. IC50 values were calculated by GraphPad Prism 6 p 0.0013. Hydroalcoholic extract showed highest presence of phenolic content (92.38☐.29 mg/g) GAE. F) Dose-response curves for parental and fulvestrant-resistant T47D and EFM-19 cells in response to 6-days treatment with increasing concentrations of the CDK1/2 inhibitor CDKi3 (100 pM to 50.
Calculated from graphs presented in (F) and in Fig.
In this tutorial I demonstrate how to plot an IC50 curve for drug assay data in Graphpad prism 6. E) CDKi3 IC50 values in parental and fulvestrant-resistant cells. ROC is a probability curve and AUC represents the degree or measure of separability. Results: Phytochemical analysis revealed the presence of rich secondary metabolite present in all the solvent extracts. Please help me to make IC50 graph with data as follow.
Statistical analysis used: Statistical analysis of data was performed by analysis of variance (one-way ANOVA) and level of statistical significance between groups was carried out using GraphPad Prism version 5.0 for Windows (GraphPad Software, San Diego, CA, USA). In vitro anticancer activity on T-24 bladder cancer cell line was assessed by MTT assay. Antioxidant potential of leaf extracts was analyzed by Ferric ion reducing antioxidant power (FRAP) assay, Phosphomolybdenum (PM) assay and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total phenolic content was estimated by Folin-Ciocalteu reagent (FCR) method. Phytochemical investigation was done using biochemical tests.
Data source location, Caguas, Puerto Rico, USA. Materials and Methods: Herbal extraction was carried out by Soxhlet method using chloroform, ethylacetate, methanol, ethanol, aqueous and hydroalcohol. Graphics and IC50 values determination were performed using GraphPad Prism 8 software. Objective: Screening of preliminary phytochemicals, evaluation of in vitro antioxidant and in vitro anticancer activities of Simarouba glauca leaf extracts on T-24 Bladder cancer cell line.